A luminescent reporter evidences active expression of Ralstonia solanacearum type III secretion system genes throughout plant infection.

@article{Monteiro2012ALR,
  title={A luminescent reporter evidences active expression of Ralstonia solanacearum type III secretion system genes throughout plant infection.},
  author={Freddy Monteiro and St{\'e}phane Genin and Irene van Dijk and Marc Valls},
  journal={Microbiology},
  year={2012},
  volume={158 Pt 8},
  pages={2107-16}
}
Although much is known about the signals that trigger transcription of virulence genes in plant pathogens, their prevalence and timing during infection are still unknown. In this work, we address these questions by analysing expression of the main pathogenicity determinants in the bacterial pathogen Ralstonia solanacearum. We set up a quantitative, non-invasive luminescent reporter to monitor in planta transcription from single promoters in the bacterial chromosome. We show that the new… CONTINUE READING
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