13,077 Citations
A routine method for embedding animal tissues in Spurr resin for electron microscopy.
- MedicineJournal of clinical pathology
- 1973
It was decided to investigate the possibility of replacing the epon mixture with Spurr's (Spurr, 1969) resin, which is considerably less viscous than epon mixtures and consequently easier to dispense.
Studies on embedding materials. I. Polydiallylphthalate (PDAP), a new embedding medium for biological tissues.
- ChemistryJournal of ultrastructure research
- 1973
The influence of embedding media and fixation on the post-embedment ultrastructural demonstration of complex carbohydrates
- BiologyHistochemistry
- 2004
Embedment of non-osmicated tissues in a styrene-Vestopal W mixture and of tissues briefly exposed to OsO4 after primary glutaraldehyde fixation in Styrene-methacrylate is recommended for optimal post-embedment DI staining of the acidic groups of complex carbohydrates.
Mercox-methylmethacrylic acid mixture penetrates into cells. A scanning and transmission electron microscopic study.
- Materials ScienceScanning microscopy
- 1993
During preparation of vascular casts using mixtures of Mercox and methylmethacrylic acid for scanning electron microscopy, occasionally irregular structures were observed adhering to otherwise well prepared corrosion casts, which indicates an unidentified less hydrophobic component is capable to infiltrate and mummify cells.
Rapid Embedding Methods into Epoxy and LR White Resins for Morphological and Immunological Analysis of Cryofixed Biological Specimens
- BiologyMicroscopy and Microanalysis
- 2013
The LR White methods offer a safer, quicker, and less-expensive alternative to Lowicryl embedding of specimens processed for on-section immunolabeling without traditional aldehyde fixatives.
GACH: a water-miscible, lipid-retaining embedding polymer for electron microscopy.
- BiologyJournal of ultrastructure research
- 1973
The influence of embedding media and fixation on the post-embedment ultrastructural demonstration of complex carbohydrates. I. Morphology and periodic acid-thiocarbohydrazide-silver proteinate staining of vicinal diols
- BiologyThe Histochemical Journal
- 2005
It was found that the combination of osmium tetroxide and epoxy resins was the least sensitive for the demonstration of complex carbohydrate with the PA-TCH-SP method.
Novel choices for formulating embedding media kits
- ChemistryScanning Microscopies
- 2009
This work examined the Flow Rate, Average Flow Rate, Volume Flow Rate, and Viscosity of Epon 812, Dodecynyl Succinic Anhydride (DDSA), Nadic Methyl Anhydride (NMA), and 2,4,6-…
Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy
- EngineeringMicroscopy research and technique
- 1993
Epoxy resins are the principal embedding media for the preservation of tissues to be sectioned and examined by transmission electron microscopy and some are toxic, they may mask antigenic sites to a greater extent than do some other embedding resins, and they do not penetrate tissues as well as less viscous embedding formulations.
The Pearson silver-gelatin method for light microscopy of 0.5-2 plastic sections.
- MedicineStain technology
- 1971
Sections 0.5-2 μ thick of liver, kidney, lung, cartilage and brown fat embedded in Maraglas, Araldrite, Epon and Spurr's medium, displayed greater clarity of cellular detail and type II cells of the lungs were exceptionally prominent.
References
SHOWING 1-10 OF 13 REFERENCES
Araldite as an Embedding Medium for Electron Microscopy
- Materials ScienceThe Journal of biophysical and biochemical cytology
- 1958
A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning, which improves the preservation of the fine structure of a variety of specimens.
Embedding in epoxy resins for ultrathin sectioning in electron microscopy.
- MedicineStain technology
- 1960
More rapid than previous techniques, this method gives blocks which do not fracture unduly on trimming and provides sections of soft tissues at 1 μ for phase contrast microscopy, as well as ultrathin sections which cut as easily with glass knives as sections of methacrylate.
Epoxy Resins in Electron Microscopy
- MedicineThe Journal of biophysical and biochemical cytology
- 1960
A method of embedding biological specimens in araldite 502 (Ciba) has been developed for materials available in the United States, and has several features—low shrinkage and absence of specimen damage during cure, minimal compression of sections, relative absence of electron beam-induced section damage, etc.—which recommends it as a routine embedding material.
A NEW MARAGLAS, D.E.R.® 732, EMBEDMENT FOR ELECTRON MICROSCOPY
- Materials ScienceThe Journal of cell biology
- 1964
The use and advantages of a new polyglycol type flexibilizer as a replacement for Cardolite NC-513 in a Maraglas 655 epoxy formulation will be presented.
TECHNICAL MODIFICATIONS IN MARAGLAS EMBEDDING
- EngineeringThe Journal of cell biology
- 1963
The embedding schedule has been modified to allow the tissue a longer period of impregnation in the Maraglas mixture prior to the final embedding and hardening, and a slight modification of Maragla mixture E as originally published to be the most reliable for biological work.
Occurrence of globular formations in thermosetting resins
- Materials Science
- 1959
Electron-microscopic examination discloses the existence of globular formations, or micelles, in thermosetting resins, varying in apparent diameter from 400–900 A. These micelles are seen in thin…
IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS
- EngineeringThe Journal of biophysical and biochemical cytology
- 1961
Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenient embedding methods for electron microscopy. The sections are robust and tissue…
Isolation of active mitochondria from tomato fruit.
- Medicine, ChemistryPlant physiology
- 1968
Electron microscope studies show that this method gives homogeneous, relatively intact mitochondria; they have a higher respiratory control ratio than those reported by other workers.
Lipid synthesis and ultrastructure of isolated barley chloroplasts.
- Environmental Science, BiologyPlant physiology
- 1968
It is suggested that the intact chloroplasts are responsible for the light induced lipid synthesis of the preparations and that the synthesizing enzymes are localized in the chloroplast stroma.
Meiosis in Coprinus lagopus: a comparative study with light and electron microscopy.
- BiologyJournal of cell science
- 1967
Meiosis within fruiting bodies of Coprinus lagopus Fr. is closely synchronized. This conveniently facilitates joint light- and electron-microscope observations. Before nuclear fusion the chromatin…