A low temperature method of isolating normal human articular chondrocytes.

  title={A low temperature method of isolating normal human articular chondrocytes.},
  author={Natasha Hidvegi and Kevin M. Sales and David Izadi and Jamie W. L. Ong and P. Kellam and David B Eastwood and P. E. M. Butler},
  journal={Osteoarthritis and cartilage},
  volume={14 1},
OBJECTIVE Numerous methods for isolation of human chondrocytes are reported in the literature, most based on isolation from animal cartilage. Normal human articular cartilage (NHAC) poses particular problems for isolating chondrocytes when compared to animal or other types of human cartilage: a hardy matrix, combined with few and friable chondrocytes makes isolation difficult. Our objective was to develop an efficient method of isolating chondrocytes from NHAC without jeopardising the viability… CONTINUE READING


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