A loop-mediated isothermal amplification method for the detection of members of the genus Ranavirus

@article{Min2013ALI,
  title={A loop-mediated isothermal amplification method for the detection of members of the genus Ranavirus},
  author={Zhang Min and Jing Hongli and Zhang Lifeng and Wang Na Na and Wu Shaoqiang and Lin Xiangmei},
  journal={Archives of Virology},
  year={2013},
  volume={158},
  pages={2121-2126}
}
A loop-mediated isothermal amplification (LAMP) method was developed for detection of members of the genus Ranavirus. The optimum reaction mixture contained 2.5 μL of each inner primer, RV-FIP (20 pmol/μL) and RV-BIP (20 pmol/μL), 0.5 μL of each outer primer, RV-F3 (10 pmol/μL) and RV-B3 (10 pmol/μL), 1.25 μL of each loop primer, RV-LF (20 pmol/μL) and RV-LB (20 pmol/μL), 3.5 μL dNTP mix (10 mM each), 8 μL MgSO4 (25 mM), 1 μL of Bst DNA polymerase (8 U/mL, large fragment; New England Biolabs… CONTINUE READING
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