A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian diary herds.

@article{Cobbold2000ALS,
  title={A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian diary herds.},
  author={Rowland N. Cobbold and Patricia M. Desmarchelier},
  journal={Veterinary microbiology},
  year={2000},
  volume={71 1-2},
  pages={
          125-37
        }
}
Over a 12 month period, 588 cattle faecal samples and 147 farm environmental samples from three dairy farms in southeast Queensland were examined for the presence of Shiga-toxigenic Escherichia coli (STEC). Samples were screened for Shiga toxin gene (stx) using PCR. Samples positive for stx were filtered onto hydrophobic grid membrane filters and STEC identified and isolated using colony hybridisation with a stx-specific DNA probe. Serotyping was performed to identify serogroups commonly… CONTINUE READING

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