A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

  title={A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.},
  author={Nancy D. Borson and Wilmar L. Salo and Lester R Drewes},
  journal={PCR methods and applications},
  volume={2 2},
We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly. 

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