A linear RNA replicon from the oomycete Phytophthora infestans

  title={A linear RNA replicon from the oomycete Phytophthora infestans},
  author={Howard S. Judelson and A.-L. Fabritius},
  journal={Molecular and General Genetics MGG},
An unusual RNA element was discovered in an isolate of the oomyceteous fungus Phytophthora infestans. The RNA exists predominantly as single-stranded molecules of about 625 nucleotides with complementary strands present at a ratio of approximately 130:1. Gel mobility and PCR assays indicated that the element was linear. The RNA appeared to be an autonomous element, since P. infestans DNA did not contain cross-hybridizing sequences. Standard methods for virus purification yielded no evidence for… CONTINUE READING
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