A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

@inproceedings{NagarkarJaiswal2015ALO,
  title={A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila},
  author={Sonal Nagarkar-Jaiswal and Pei-Tseng Lee and Megan E Campbell and Kuchuan Chen and Stephanie S. Anguiano-Zarate and Manuel Cantu Gutierrez and Theodore Busby and Wen-wen Lin and Yuchun He and Karen L Schulze and Benjamin W. Booth and Martha Evans-Holm and Koen J T Venken and Robert W. Levis and Allan C Spradling and Roger A. Hoskins and Hugo J. Bellen},
  booktitle={eLife},
  year={2015}
}
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes… CONTINUE READING
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