A lavage technique allowing repeated measurement of IgA antibody in mouse intestinal secretions.

@article{Elson1984ALT,
  title={A lavage technique allowing repeated measurement of IgA antibody in mouse intestinal secretions.},
  author={Charles O Elson and W Ealding and Jeff Lefkowitz},
  journal={Journal of immunological methods},
  year={1984},
  volume={67 1},
  pages={101-8}
}
Mouse intestinal secretions can be readily obtained without harm to the mice by administering a lavage solution to them intragastrically followed by pilocarpine intraperitoneally. These secretions are rich in proteases but this enzyme activity can be blocked by addition of a mixture of inhibitors. Both total and specific IgA antibody could be measured in these secretions using ELISA techniques. The total IgA recovered was found to vary considerably, even in the same group of mice sampled on… CONTINUE READING

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