A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella.

@article{Gibbons1979ALA,
  title={A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella.},
  author={Ian R. Gibbons and E. Fronk},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 1},
  pages={187-96}
}
Treatment of demembranated sea urchin sperm axonemes with an extraction solution containing 0.6 M NaCl, pH 7.0 for 10 min at 4 degrees C yields a solution of dynein 1 having a low, latent specific ATPase activity of about 0.25 mumol of Pi mg(-1) min(-1). Exposure of this dynein solution to 0.1% Triton-X-100 for 10 min at 25 degrees C causes an increase in its ATPase activity to about 3 mumol of Pi mg(-1) min(-1). A similar activation can be obtained by treating at 42 degrees C or by reacting… CONTINUE READING
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