A large case-control study of cervical cancer risk associated with human papillomavirus infection in Japan, by nucleotide sequencing-based genotyping.

@article{Asato2004ALC,
  title={A large case-control study of cervical cancer risk associated with human papillomavirus infection in Japan, by nucleotide sequencing-based genotyping.},
  author={Tsuyoshi Asato and Toshiyuki Maehama and Yutaka Nagai and Koji Kanazawa and Hiroshi Uezato and Ken-ichi Kariya},
  journal={The Journal of infectious diseases},
  year={2004},
  volume={189 10},
  pages={1829-32}
}
Using nucleotide sequencing-based genotyping, we conducted a case-control study to examine cervical cancer risk associated with human papillomavirus (HPV) infection in a Japanese population. A consensus primer pair was used to amplify DNA from the L1 region of HPV by polymerase chain reaction (PCR). By PCR, 311 of 356 patients with cervical cancer and 333 of 3249 control individuals were positive for HPV. By the direct sequencing of PCR products, nucleotide sequences of 30 genotypes were… CONTINUE READING
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