A labile point in mutant amphotericin polyketide synthases

@article{Khan2011ALP,
  title={A labile point in mutant amphotericin polyketide synthases},
  author={Naseem Khan and Bernard J. Rawlings and Patrick Caffrey},
  journal={Biotechnology Letters},
  year={2011},
  volume={33},
  pages={1121-1126}
}
Streptomyces nodosus produces the antifungal polyene amphotericin B. Numerous modifications of the amphotericin polyketide synthase have yielded new analogues. However, previous inactivation of the ketoreductase in module 10 resulted in biosynthesis of truncated polyketides. Here we show that modules downstream of this domain remain intact. Therefore, loss of ketoreductase-10 activity is sufficient to cause early chain termination. This modification creates a labile point in cycle 11 of the… 
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