A label-free method for detecting biological thiols based on blocking of Hg2+-quenching of fluorescent gold nanoclusters.

@article{Kim2013ALM,
  title={A label-free method for detecting biological thiols based on blocking of Hg2+-quenching of fluorescent gold nanoclusters.},
  author={Moon Il Kim and Min-Ah Woo and Hyun Gyu Park},
  journal={Biosensors & bioelectronics},
  year={2013},
  volume={45},
  pages={
          65-9
        }
}
A novel, label-free, fluorescent, turn-on sensor for biological thiol detection that uses highly fluorescent gold nanoclusters (AuNCs), prepared by a bovine serum albumin (BSA)-templated green synthetic route, has been developed. The assay relies on blocking Hg(2+)-induced quenching of the fluorescence of AuNCs, caused by metallophilic Hg(2+)-Au(+) interactions, through selective coordination of biological thiols with Hg(2+) ions. Biological thiols entrap added Hg(2+) ions via a robust Hg-S… CONTINUE READING
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