A kinetic comparison of asparagine synthetase isozymes from higher plants.

  title={A kinetic comparison of asparagine synthetase isozymes from higher plants.},
  author={Stephen M. G. Duff and Qungang Qi and Timothy Reich and Xiaoyun Wu and Todd C Brown and James H Crowley and Brad Fabbri},
  journal={Plant physiology and biochemistry : PPB},
  volume={49 3},
Four previously identified maize asparagine synthetase (AsnS) genes and a soy AsnS gene have been cloned and expressed in Escherichia coli. The enzymes have been purified and kinetically characterized. The plant AsnS proteins were expressed mainly in the inclusion bodies although small amounts of one form (ZmAsnS2) were recovered in the soluble fraction. In order to measure the kinetic properties of these enzymes a sensitive assay based on the detection of Asn by HPLC has been developed. In… CONTINUE READING


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