A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR

@article{Rutledge2008AKS,
  title={A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR},
  author={R G Rutledge and Don Stewart},
  journal={BMC Biotechnology},
  year={2008},
  volume={8},
  pages={47 - 47}
}
BACKGROUND Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construction of target-specific standard curves that are highly resource intensive and prone to introducing quantitative errors. Sigmoidal modeling using nonlinear regression has previously demonstrated that absolute quantification can be accomplished without standard curves; however… CONTINUE READING

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Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction

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