A hyperglycemic peptide from pituitary growth hormone: preparation with pepsin and assay in ob-ob mice.

Abstract

A fragment that induces glucose intolerance in hereditarily obese mice has been prepared from pituitary growth hormone(sheep and human) by controlled digestion with pepsin in 0.05 M sodium acetate buffer, pH 3.7. The digest was purified by column chromatography on Bio-Gel P-6, followed by chromatography on Bio-Rex 70 and Bio-Gel P-2; in each successive step… (More)

Topics

3 Figures and Tables

Slides referencing similar topics