Excerpta Medica Foundation (International Congress Series
- C. H. Li, H. M. Evans, M. E. Simpson
- J. Biol. Chem
- 1973
A hyperglycemic peptide from pituitary growth hormone: preparation with pepsin and assay in ob-ob mice.
- Published 1974 in Proceedings of the National Academy of Sciences…
Abstract
A fragment that induces glucose intolerance in hereditarily obese mice has been prepared from pituitary growth hormone(sheep and human) by controlled digestion with pepsin in 0.05 M sodium acetate buffer, pH 3.7. The digest was purified by column chromatography on Bio-Gel P-6, followed by chromatography on Bio-Rex 70 and Bio-Gel P-2; in each successive step… (More)