A hybrid approach to measuring electrical activity in genetically specified neurons

@article{Chanda2005AHA,
  title={A hybrid approach to measuring electrical activity in genetically specified neurons},
  author={Baron Chanda and Rikard Blunck and Leonardo Coutinho Faria and Felix Erich Schweizer and Istv{\'a}n M{\'o}dy and Francisco Bezanilla},
  journal={Nature Neuroscience},
  year={2005},
  volume={8},
  pages={1619-1626}
}
The development of genetically encoded fluorescent voltage probes is essential to image electrical activity from neuronal populations. Previous green fluorescent protein (GFP)-based probes have had limited success in recording electrical activity of neurons because of their low sensitivity and poor temporal resolution. Here we describe a hybrid approach that combines a genetically encoded fluorescent probe (membrane-anchored enhanced GFP) with dipicrylamine, a synthetic voltage-sensing molecule… CONTINUE READING
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