A human single chain transbody specific to matrix protein (M1) interferes with the replication of influenza A virus.

@article{Poungpair2010AHS,
  title={A human single chain transbody specific to matrix protein (M1) interferes with the replication of influenza A virus.},
  author={Ornnuthchar Poungpair and Anek Pootong and Santi Maneewatch and Potjanee Srimanote and Pongsri Tongtawe and Thaweesak Songserm and Pramuan Tapchaisri and Wanpen Chaicumpa},
  journal={Bioconjugate chemistry},
  year={2010},
  volume={21 7},
  pages={1134-41}
}
A cell penetrating format of human single chain antibody (HuScFv) specific to matrix protein (M1) of influenza A virus was produced by molecular linking of the gene sequence encoding the HuScFv (huscfv) to a protein transduction domain, i.e., penetratin (PEN) of the Drosophila homeodomain. DNA of a recombinant phagemid vector carrying the huscfv was used as a platform template in a three-step PCR for generating a nucleotide sequence encoding a 16 amino acid PEN peptide. The PEN-HuScFv had… CONTINUE READING