A human protein containing a "cold shock" domain binds specifically to H-DNA upstream from the human gamma-globin genes.

@article{Horwitz1994AHP,
  title={A human protein containing a "cold shock" domain binds specifically to H-DNA upstream from the human gamma-globin genes.},
  author={Edwin Mark Horwitz and K A Maloney and Timothy J. Ley},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 19},
  pages={
          14130-9
        }
}
We previously determined that a region between positions -228 and -189 upstream from the human gamma-globin genes can form an intramolecular triplex (H-DNA) in supercoiled plasmids. To identify proteins that might interact with this DNA structure, we performed expression cloning using an adult bone marrow cDNA library and the single-stranded region of the H-DNA structure as a probe. We cloned molecules very similar to two previously identified cDNAs, dbpA and dbpB. The dbpB-like protein (called… CONTINUE READING

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