A human hepatoma cell line expressing hepatitis c virus nonstructural proteins tightly regulated by tetracycline.

@article{Huang2001AHH,
  title={A human hepatoma cell line expressing hepatitis c virus nonstructural proteins tightly regulated by tetracycline.},
  author={Yun Wen Huang and Yasuo Uchiyama and Tsutomu Fujimura and Hisayuki Kanamori and Takefumi Doi and Akihisa Takamizawa and Takao Hamakubo and Tatsuhiko Kodama},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={281 3},
  pages={
          732-40
        }
}
Nonstructural (NS) proteins of hepatitis C virus (HCV) play major roles in viral replication and the pathogenesis of liver diseases. Current studies on antiviral strategies targeting these proteins have been hampered by the lack of efficient cell culture systems. Combining tetracycline-regulated gene expressing system and enhanced green fluorescent protein (EGFP), we generated a human hepatoma cell line inducibly expressing the HCV NS proteins. This cell line exhibited high induction of a full… CONTINUE READING
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An overview: in vitro models of HCV replication in different cell cultures.

  • Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2012
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