• Corpus ID: 892621

A human hepatocellular carcinoma 3.0-kilobase DNA sequence transforms both rat liver cells and NIH3T3 fibroblasts and encodes a 52-kilodalton protein.

@article{Yang1990AHH,
  title={A human hepatocellular carcinoma 3.0-kilobase DNA sequence transforms both rat liver cells and NIH3T3 fibroblasts and encodes a 52-kilodalton protein.},
  author={S S Yang and K E Zhang and William Viera and Janet Taub and Jill Zeilstra-Ryalls and Ronald L. Somerville},
  journal={Cancer research},
  year={1990},
  volume={50 17 Suppl},
  pages={
          5658S-5667S
        }
}
Neoplastic transformation of rat liver cells in vitro by DNA-mediated gene transfer with an oncogene, hhcM, derived from human (Mahlavu) hepatocellular carcinoma, is described and compared with that of NIH3T3 cells. hhcM was cloned in a neomycin-resistant simian virus 40 promoter vector (pNeor/S) and was designated pNrpM-1. BRL-1 or NIH3T3 cells, transfected with pNrpM-1 DNA, showed significant morphological changes, loss of contact inhibition, and anchorage-independent growth. They became… 
3 Citations
Increased growth of NIH/3T3 cells by transfection with human p120 complementary DNA and inhibition by a p120 antisense construct.
TLDR
The human nucleolar antigen p120 was detected with an anti-p120 monoclonal antibody in most human malignant tumors but not in most resting human tissues and has been used as a prognostic tumor marker in breast cancer patients.
The stem cells of the liver — a selective review
  • K. Aterman
  • Medicine, Biology
    Journal of Cancer Research and Clinical Oncology
  • 2005
TLDR
It appears that the evidence in favour of the stem-cell hypothesis is still circumstantial and that the hepatocytic theory has not been invalidated, presumably the question of the hepatic stem cells will be answered when the riddle of hepatocarcino-genesis has been solved.
Current pathogenetic and molecular concepts in viral liver carcinogenesis
TLDR
Improved knowledge of the molecular biology of HBV has led to better understanding of its pleiotropic effects on induction and progression in hepatocarcinogenesis, and new insights are gained into how these factors are activated and may interact.

References

SHOWING 1-10 OF 25 REFERENCES
Transforming DNA sequences of human hepatocellular carcinomas, their distribution and relationship with hepatitis B virus sequence in human hepatomas.
TLDR
The results suggest that HBV contributes to hepatocarcinogenesis probably via an activation mechanism involving possibly an integration or transient interaction of HBV DNA with hepatocyte DNA sequences, leading to recombination and eventual amplifications of the hhcM sequence in Mahlavu.
Tumorigenicity of simian virus 40-hepatocyte cell lines: effect of in vitro and in vivo passage on expression of liver-specific genes and oncogenes.
TLDR
A model system is developed with five SV40-hepatocyte cell lines, tumors induced by them, and tumor cell lines to examine changes in gene expression that accompany the progression from a normal cell to a hepatocellular carcinoma.
Molecular cloning of the endogenous rat C-type helper virus DNA sequence: structural organization and functional analysis of some restricted DNA fragments.
TLDR
All major RaLV-specific proteins precipitable by anti-RaLV serum were synthesized in vitro, confirming that the RHHV genomic DNA was successfully cloned with little fidelity loss or scrambling of the genetic information.
Dose dependency of aflatoxin B1 binding on human high molecular weight DNA in the activation of proto-oncogene.
TLDR
Excessive AFB1 binding on the hHC and PLC HMW DNAs resulted in an "over-kill" of both cell transformation capability and templating activity of the DNA.
Oncogenes in solid human tumours
TLDR
It is reported here that unmanipulated human solid tumours, including carcinomas of the colon, lung and pancreas and an embryonal rhabdomyosarcoma, also contain dominant transforming genes, and several other human tumour cell lines, including those established from carcinomasOf the colon (A2233), lung (A427 and A2182), gall bladder and urinary bladder, possess the same oncogene.
Transformation of differentiated neonatal rat hepatocytes in primary culture by polyoma virus early region sequences.
TLDR
It is demonstrated that a proto-oncogene was activated after transfection of hepatocytes with DNA tumor virus transforming genes, however, the expression of c-neu oncogene is not related to the maintenance of the transformed state.
Transformation of differentiated rat hepatocytes with adenovirus and adenovirus DNA
TLDR
It is concluded that the adenovirus E1A and E1B genes are capable of transforming adult rat hepatocytes, a differentiated epithelial cell type.
...
...