A human endometrial explant system: validation and potential applications.

  title={A human endometrial explant system: validation and potential applications.},
  author={Donald J. Dudley and Harry H. Hatasaka and D Ware Branch and Elizabeth H. Hammond and Murray D. Mitchell},
  journal={American journal of obstetrics and gynecology},
  volume={167 6},
Critical evaluation of human endometrial explants as an ex vivo model system: a molecular approach.
It is concluded that human endometrial explants are a complex model due to limited viability, difficult standardization and intrinsic alterations during culture.
Morphology of human endometrial explants and secretion of stromal marker proteins in short- and long-term cultures
It is concluded that the explant culture model is viable for not more than 48 h in vitro for stromal cells, but that this interval can be prolonged by the addition of progesterone which initiates decidualisation.
Development of A 3D Tissue Slice Culture Model for the Study of Human Endometrial Repair and Regeneration
A robust new method to study endometrial regeneration and differentiation processes using human endometrian tissue slice cultures incorporating an air-liquid interface into a 3D matrix scaffold of type I collagen gel, allowing sustained tissue viability over three weeks.
The effect of RU486 on the gene expression profile in an endometrial explant model.
Results show that components of two important signalling pathways in endometrium-the JAK/STAT pathway, and the JNK pathway-are altered by RU486.
Amniotic Membrane as an in vitro Model for Endometrium- Extracellular Matrix Interactions
An in vitro model to study the interaction between endometrial cells and extracellular matrix (ECM) and to evaluate the expression of cell adhesion molecules in endometrian cells and tissue fragments under in vitro conditions is developed.
TWEAK Appears as a Modulator of Endometrial IL-18 Related Cytotoxic Activity of Uterine Natural Killers
In a pro-inflammatory environment (obtained by an excess of IL-18), inhibition of TWEAK was able to increase significantly NKp46 and TGF-beta1 mRNA expressions, and appears as a crucial physiological modulator to prevent endometrial uNK cytotoxicity in human.
Triphasic pattern in the ex vivo response of human proliferative phase endometrium to oestrogens


Prostaglandin secretion by human endometrium in vitro.
  • B. Tsang, T. Ooi
  • Biology, Medicine
    American journal of obstetrics and gynecology
  • 1982
Prostaglandin biosynthesis by human decidual cells: effects of inflammatory mediators.
Organ Culture of Human Endometrium: Effects of Progesterone
The anticipated secretory differentiative alterations of the progesterone-stimulated explants took place irrespective of the cycle day of origin of the endometrium, and were associated with augmented alkaline phosphatase activity of the glandular cells.
In vitro effects of ovarian steroids on prostaglandin F2 alpha output by human endometrium and endometrial epithelial cells.
The results from this study confirm that only secretory endometrium responds to E2 in vitro by significantly increasing PGF2 alpha output, and suggest that an inhibitory influence is removed during the isolation or subsequent culture of endometrial glands.
Prostaglandin production by intra-uterine tissues from periparturient sheep: use of a superfusion technique.
Oxytocin had no effect on the production of prostaglandins by tissues obtained before labour but myometrium and maternal cotyledon obtained at delivery exhibited a significant increase in production of PGE and PGF (though not PGFM) in response to oxytocin.
Activation and distribution of inflammatory cells in the mouse uterus during the preimplantation period.
The results strongly suggest a role for some factor in the ejaculate, other than spermatozoa, in the initiation of a uterine inflammatory response after mating, but an effect of the act of mating cannot be excluded.
Cytokine-induced production of IFN-beta 2/IL-6 by freshly explanted human endometrial stromal cells. Modulation by estradiol-17 beta.
The possibility that stromal cell secreted IFN-beta 2/IL-6 may affect the physiology of the overlying epithelium in an hormonally modulated manner is suggested.
Stimulation of rheumatoid synovial cell collagenase and prostaglandin production by partially purified lymphocyte-activating factor (interleukin 1).
The results of these studies have raised the possibility that LAF (IL 1) may play a role in macrophage-mediated activation of synovial cells and lymphocytes which are involved in the inflammatory responses associated with rheumatoid arthritis.