A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains

  title={A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains},
  author={Yusuke Yanagi and Yasunobu Yoshikai and Kathleen Leggett and S. P. Clark and Ingrid Aleksander and Tak Wah Mak},
We have cloned and sequenced a human mRNA specific for mammalian T-lymphoid cells. The message was found to be expressed in human and murine T lymphoblasts, thymocytes and phytohaemagglutinin-stimulated T lymphocytes. The protein deduced from the cDNA sequence has a molecular weight of 34,938 and shows extensive similarity to the entire length of the variable, joining and constant regions of mammalian immunoglobulin light chains. In addition, the relative positions of the cysteine residues are… 

Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex

The complete amino acid sequence of one of the polypeptide chains of the human T3/T-cell receptor complex, the T3 glycoprotein (T3-δ-chain) was deduced from cDNA clones derived from HPB-ALL cells and a human T-cell clone.

Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences

Two related, but distinct cDNA clones have been isolated and sequenced from a functional murine cytotoxic T-lymphocyte clone and it is concluded that these genes code for the two subunits of the heterodimeric antigen receptor on the surface of the T cell.

A third type of murine T-cell receptor gene

A new species of T-cell receptor cDNA clone whose predicted amino acid sequence has homology to variable, constant, joining and diversity segments of immunoglobulins and T- cell receptors is isolated.

Molecular Studies of the Human T-Cell Antigen Receptor

The mouse and human cDNA probes most probably detect the β chain family of the T- cell antigen receptor since partial protein sequence analysis of a human T-cell β chain agrees with the translated nucleotide sequence of the human c DNA clone.

Sequence and organization of the human T cell δ chain gene

This gene undergoes rearrangements and is expressed at the RNA level in human thymocytes, peripheral T cells and several leukemic T cell lines which have been shown to express the surface γ‐δ heterodimer, suggesting that this gene encodes the human T cell δ chain.

The complete primary structure of the T -Cell receptor genes from an alloreactive cytotoxic human T-lymphocyte clone

Sequence analysis of the cDNAs encoding the α and β chains of the T-lymphocyte receptor for antigen from a human alloreactive, cytotoxic T-cell clone, L17, reveals that both genes are related to immunoglobulins and are composed of variable, diversity, and constant region sequences.



Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.

Several genes for the variable region of immunoglobulin heavy chains (VH genes) have been isolated from human fetal liver DNA by using a cDNA plasmid probe containing a mouse VH sequence, suggesting that similar enzymatic mechanisms are involved in the integration of V genes in both heavy and light chains.

Thy-1 cDNA sequence suggests a novel regulatory mechanism

The isolation and characterization of a cDNA clone encoding the rat thymus Thy-1 antigen is reported but it is found that the DNA sequence ends prematurely at a position corresponding to amino acid 103, which appears to be a complete transcript.

Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.

  • J. ParnesB. Velan J. Seidman
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
The positive-selection--hybridization assay has been modified to facilitate the screening of large numbers of cDNA clones, and the modified assay should allow the isolation of cDNAs corresponding to any mRNA whose in vitro translation products can be immunoprecipitated.

Structure of C-terminal half of two H–2 antigens from cloned mRNA

The sequencing of plasmids carrying H–2 cDNA is reported as a means of inferring the amino acid sequence of the antigens, and especially of their previously poorly described C-terminal half.

Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.

  • N. HozumiS. Tonegawa
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1976
The results were interpreted to mean that the Vk and Ck genes are joined to form a contiguous polynucleotide stretch during differentiation of lymphocytes, which occurs in both of the homologous chromosomes.