A human ESC model for MLL-AF4 leukemic fusion gene reveals an impaired early hematopoietic-endothelial specification

@article{Bueno2012AHE,
  title={A human ESC model for MLL-AF4 leukemic fusion gene reveals an impaired early hematopoietic-endothelial specification},
  author={Clara Bueno and Rosa Montes and Gustavo J. Melen and Ver{\'o}nica Ramos-Mej{\'i}a and Pedro J Real and Ver{\'o}nica Ayll{\'o}n and L F Cerd{\'a}n S{\'a}nchez and Gertrudis Ligero and Iv{\'a}n Guti{\'e}rrez-Aranda and Agust{\'i}n F Fern{\'a}ndez and Mario F Fraga and Inmaculada Moreno-Gimeno and Deborah M Burks and Mar{\'i}a del Carmen Plaza-Calonge and Juan Carlos Rodr{\'i}guez-Manzaneque and P. J. Men{\'e}ndez},
  journal={Cell Research},
  year={2012},
  volume={22},
  pages={986-1002}
}
The MLL-AF4 fusion gene is a hallmark genomic aberration in high-risk acute lymphoblastic leukemia in infants. Although it is well established that MLL-AF4 arises prenatally during human development, its effects on hematopoietic development in utero remain unexplored. We have created a human-specific cellular system to study early hemato-endothelial development in MLL-AF4-expressing human embryonic stem cells (hESCs). Functional studies, clonal analysis and gene expression profiling reveal that… CONTINUE READING