A homologous radioimmunoassay for rat insulin-like growth factor-I (IGF-I): implications for studies of human IGF-I physiology.

@article{Lee1996AHR,
  title={A homologous radioimmunoassay for rat insulin-like growth factor-I (IGF-I): implications for studies of human IGF-I physiology.},
  author={P. D. Lee and Bonita K. Baker and Francis Liu and Eugene Kwan and Raymond L. Hintz},
  journal={The Journal of clinical endocrinology and metabolism},
  year={1996},
  volume={81 5},
  pages={2002-5}
}
We report the development of a unique radioimmunoassay (RIA) for rat insulin-like growth factor-I (rIGF-I) which does not recognize human IGF-I (hIGF-I). The rIGF RIA uses a specific anti-rIGF-I antiserum with rIGF-I standards and radioligand. Rat sera were extracted by either an abbreviated acid/ethanol procedure or by acid-chromatography with virtually identical results. Assay sensitivity is congruent to 0.5 ng/tube and inter- and intra-assay coefficients of variation were 3.4-7.6% and 4.8-9… CONTINUE READING

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