A homogeneous high-throughput genotyping method based on competitive hybridization.

@article{Kiviniemi2003AHH,
  title={A homogeneous high-throughput genotyping method based on competitive hybridization.},
  author={M. Kiviniemi and Jussi T Nurmi and Hannu Turpeinen and Timo L{\"o}vgren and Jorma Ilonen},
  journal={Clinical biochemistry},
  year={2003},
  volume={36 8},
  pages={633-40}
}
OBJECTIVES A reliable high-throughput assay system is necessary for the analysis of the ever-increasing numbers of single-nucleotide polymorphisms (SNP) relevant to genetic screening studies. We describe an assay suitable also for large-scale screening programs. DESIGN AND METHODS The one-step assay is based on asymmetric PCR amplification of the target sequence and subsequent time-resolved fluorescence measurement. Asymmetric amplification results in a single-stranded PCR product that is… CONTINUE READING