A highly stable polyethylene glycol-conjugated human single-chain antibody neutralizing granulocyte-macrophage colony stimulating factor at low nanomolar concentration.

@article{Krinner2006AHS,
  title={A highly stable polyethylene glycol-conjugated human single-chain antibody neutralizing granulocyte-macrophage colony stimulating factor at low nanomolar concentration.},
  author={Eva-Maria Krinner and Julia Hepp and Patrick Hoffmann and Sandra Bruckmaier and Laetitia Petersen and Silke Petsch and Larissa Parr and Ioana Schuster and Susanne Mangold and Grit Lorenczewski and Petra Lutterbuese and Stefan Buziol and Inessa Hochheim and J{\"o}rg Volkland and Michael M{\o}lh{\o}j and Mirnalini Sriskandarajah and Markus Stra{\ss}er and Christian Itin and Andreas Wolf and Amartya Basu and Karen Yang and David R. Filpula and Poul Ejnar S{\o}rensen and Peter Kufer and Patrick Baeuerle and Tobias Raum},
  journal={Protein engineering, design & selection : PEDS},
  year={2006},
  volume={19 10},
  pages={
          461-70
        }
}
GM-CSF (granulocyte-macrophage colony stimulating factor) plays a central role in inflammatory processes. Treatment with antibodies neutralizing murine GM-CSF showed significant therapeutic effects in mouse models of inflammatory diseases. We constructed by phage display technology a human scFv, which could potently neutralize human GM-CSF. At first, a human V(L) repertoire was combined with the V(H) domain of a parental GM-CSF-neutralizing rat antibody. One dominant rat/human scFv clone was… CONTINUE READING

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Humanization of antibodies.

  • Frontiers in bioscience : a journal and virtual library
  • 2008
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