A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.

@article{McCraith1990AHS,
  title={A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.},
  author={Stephen M McCraith and Eric M Phizicky},
  journal={Molecular and cellular biology},
  year={1990},
  volume={10 3},
  pages={1049-55}
}
We identified and partially purified a phosphatase from crude extracts of Saccharomyces cerevisiae cells that can catalyze the last step of tRNA splicing in vitro. This phosphatase can remove the 2'-phosphate left over at the splice junction after endonuclease has removed the intron and ligase has joined together the two half-molecules. We suggest that this phosphatase is responsible for the completion of tRNA splicing in vivo, based primarily on its specificity for the 2'-phosphate of spliced… CONTINUE READING

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