A highly sensitive radioimmunoassay of atrial natriuretic peptide (ANP) in human plasma and urine.

@article{Marumo1986AHS,
  title={A highly sensitive radioimmunoassay of atrial natriuretic peptide (ANP) in human plasma and urine.},
  author={Fumiaki Marumo and Hisato Sakamoto and Katsuyuki Ando and Tomoaki Ishigami and Makoto Kawakami},
  journal={Biochemical and biophysical research communications},
  year={1986},
  volume={137 1},
  pages={231-6}
}
A highly sensitive radioimmunoassay has been established for measurement of human plasma and urine concentrations of atrial natriuretic peptide (ANP) and requires no extraction or concentration process such as Sep-Pak C-18 cartridge treatment. An antiserum was prepared from rabbits immunized with alpha-human ANP (alpha-hANP) coupled with bovine-thyroglobulin. The sensitivity of this method was 0.3 pg/tube of synthetic alpha-hANP utilized as authentic standard. Recovery of alpha-hANP spiked to… CONTINUE READING

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