A highly sensitive high-performance liquid chromatography method for the estimation of ascorbic and dehydroascorbic acid in tissues, biological fluids, and foods.

@article{Behrens1987AHS,
  title={A highly sensitive high-performance liquid chromatography method for the estimation of ascorbic and dehydroascorbic acid in tissues, biological fluids, and foods.},
  author={W. Behrens and R. Mad{\`e}re},
  journal={Analytical biochemistry},
  year={1987},
  volume={165 1},
  pages={
          102-7
        }
}
A highly sensitive procedure for determining ascorbic acid (AA) and dehydroascorbic acid (DHAA) by high-performance liquid chromatography with electrochemical detection in biological fluids, tissues, and foods is described. AA is separated in a C18 reverse-phase column after extraction from the sample with metaphosphoric acid. An aliquot of 20 microliter of diluted extract is injected into the column for the estimation of AA. DHAA is indirectly estimated by converting it to AA after reduction… Expand
186 Citations
Determination of ascorbic and dehydroascorbic acids in blood plasma and serum by liquid chromatography.
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References

SHOWING 1-10 OF 11 REFERENCES
Improved automated method for determining vitamin C in plasma and tissues.
  • 10
SYNTHESIS AND SOME MAJOR FUNCTIONS OF VITAMIN C IN ANIMALS *
  • 332
The use of homocysteine in the estimation of dehydroascorbic acid.
  • R. Hughes
  • Chemistry, Medicine
  • The Biochemical journal
  • 1956
  • 117
  • PDF
Blood Dehydroascorbic Acid and Diabetes Mellitus in Human Beings
  • A. Banerjee
  • Biology, Medicine
  • Annals of clinical biochemistry
  • 1982
  • 43
DISTRIBUTION OF ASCORBIC ACID, METABOLITES AND ANALOGUES IN MAN AND ANIMALS
  • D. Hornig
  • Chemistry, Medicine
  • Annals of the New York Academy of Sciences
  • 1975
  • 397
An increase in vitamin E requirement induced by high supplementation of vitamin C in rats.
  • L. Chen
  • Chemistry, Medicine
  • The American journal of clinical nutrition
  • 1981
  • 61
Metabolism and transport of dehydroascorbic acid in erythrocytes of "spontaneous diabetic BB/W" Wistar rats.
  • R. C. Rose
  • Biology, Medicine
  • Metabolism: clinical and experimental
  • 1986
  • 12
...
1
2
...