A highly sensitive and multiplexed method for focused transcript analysis.

@article{Kataja2006AHS,
  title={A highly sensitive and multiplexed method for focused transcript analysis.},
  author={Kari Kataja and Reetta M. Satokari and Mikko Arvas and Kristiina Takkinen and Hans S{\"o}derlund},
  journal={Journal of microbiological methods},
  year={2006},
  volume={67 1},
  pages={102-13}
}
We describe a novel, multiplexed method for focused transcript analysis of tens to hundreds of genes. In this method TRAC (transcript analysis with aid of affinity capture) mRNA targets, a set of amplifiable detection probes of distinct sizes and biotinylated oligo(dT) capture probe are hybridized in solution. The formed sandwich hybrids are collected on magnetic streptavidin-coated microparticles and washed. The hybridized probes are eluted, optionally amplified by a PCR using a universal… CONTINUE READING