A highly sensitive HPLC-MS/MS method for quantification of 20(S)-protopanaxadiol in human plasma and its application in phase IIa clinical trial of a novel antidepressant agent.

@article{Ling2016AHS,
  title={A highly sensitive HPLC-MS/MS method for quantification of 20(S)-protopanaxadiol in human plasma and its application in phase IIa clinical trial of a novel antidepressant agent.},
  author={Jin Ling and Yingjia Yu and Jiajun Zhu and Yan Li and Li Ling and Liping Wang and Changjiang Xu and Gengli Duan},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2016},
  volume={1031},
  pages={
          214-220
        }
}
A highly sensitive HPLC-MS/MS assay method was established to quantify 20(S)-protopanaxadiol (PPD) in human plasma with dexamethasone as an internal standard. The electrospray ion mass spectrometry (ESI-MS) was operated under the multiple reactions monitoring mode (MRM) using positive ion mode. PPD was extracted from 500μL plasma samples by liquid-liquid extraction then separated by a C18 analytical column with gradient elution. The concentration of PPD could be determined by this HPLC-MS/MS… CONTINUE READING