A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars.

@article{Quint2007AHS,
  title={A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars.},
  author={Koen Dirk Quint and Leen-Jan van Doorn and Bernhard Kleter and Maurits N. C. de Koning and Henk A M van den Munckhof and Servaas A Morr{\'e} and Bram ter Harmsel and Elisabete Weiderpass and Gonneke K Harbers and Willem J G Melchers and Wim G. V. Quint},
  journal={The Journal of molecular diagnostics : JMD},
  year={2007},
  volume={9 5},
  pages={631-8}
}
Chlamydia trachomatis (Ct) comprises distinct serogroups and serovars. The present study evaluates a novel Ct amplification, detection, and genotyping method (Ct-DT assay). The Ct-DT amplification step is a multiplex broad-spectrum PCR for the cryptic plasmid and the VD2-region of ompl. The Ct-DT detection step involves a DNA enzyme immunoassay (DEIA) using probes for serogroups (group B, C, and intermediate) and the cryptic plasmid, permitting sensitive detection of 19 Ct serovars (A, B/Ba, C… CONTINUE READING
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