A highly salt-dependent enthalpy change for Escherichia coli SSB protein-nucleic acid binding due to ion-protein interactions.

@article{Lohman1996AHS,
  title={A highly salt-dependent enthalpy change for Escherichia coli SSB protein-nucleic acid binding due to ion-protein interactions.},
  author={Timothy M. Lohman and Leslie B. Overman and Marylin E Ferrari and Alexander G. Kozlov},
  journal={Biochemistry},
  year={1996},
  volume={35 16},
  pages={5272-9}
}
We have examined the linkage between salt concentration and temperature for the equilibrium binding of the tetrameric Escherichia coli single-stranded binding (SSB) protein to three single-stranded nucleic acids, poly(U), dA(pA)69, and dT(pT)69, by van't Hoff analysis and isothermal titration calorimetry (ITC). For SSB binding to poly(U) in its (SSB)65 mode… CONTINUE READING