A highly flexible tRNA acylation method for non-natural polypeptide synthesis

  title={A highly flexible tRNA acylation method for non-natural polypeptide synthesis},
  author={Hiroshi Murakami and Atsushi Ohta and Hiroshi Ashigai and Hiroaki Suga},
  journal={Nature Methods},
Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.*Note: In the version of this article initially published, the authors did not declare competing financial… CONTINUE READING


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