A highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and Tween 80.

@article{Armon1988AHE,
  title={A highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and Tween 80.},
  author={R Armon and Maximilen Arella and Pierre Payment},
  journal={Canadian journal of microbiology},
  year={1988},
  volume={34 5},
  pages={651-5}
}
Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obtained with several nonionic detergents including Tween 80 and under a salt saturation of 55% with ammonium sulfate, potassium tartrate, and sodium phosphate. Virus recovery ranged… CONTINUE READING