A highly efficient polyethylene glycol-mediated transformation method for mushrooms.

@article{Li2006AHE,
  title={A highly efficient polyethylene glycol-mediated transformation method for mushrooms.},
  author={Gang Li and Ruixue Li and Qiuyun Liu and Qiang Wang and Min Chen and Baojian Li},
  journal={FEMS microbiology letters},
  year={2006},
  volume={256 2},
  pages={203-8}
}
A highly efficient transformation system mediated by polyethylene glycol was developed for the cultivated mushroom Pleurotus ostreatus. Eighty to 180 integrative and stable-resistant colonies appeared per mug of DNA per 10(7) viable protoplasts in a transformation experiment with the hygromycin B phosphotransferase gene (hph), which is about 40-1800 times higher than that previously reported in P. ostreatus. One hundred to 150 transformants emitting green fluorescence were observed per mug of… CONTINUE READING