A highly efficient maize nucellus protoplast system for transient gene expression and studying programmed cell death-related processes

Abstract

Conditions for the isolation and transfection of maize nucellus protoplasts were established. We demonstrated its utilization for protein expression, localization, protein–protein interaction, and the investigation of PCD-related processes. Plant protoplasts are an important and versatile cell system that is widely used in the analysis of gene… (More)
DOI: 10.1007/s00299-015-1783-z

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Cite this paper

@article{Chen2015AHE, title={A highly efficient maize nucellus protoplast system for transient gene expression and studying programmed cell death-related processes}, author={Jiang Chen and Qiang Yi and Qiaoheng Song and Yong Gu and Junjie Zhang and Yufeng Hu and Hanmei Liu and Yinghong Liu and Guowu Yu and Yubi Huang}, journal={Plant Cell Reports}, year={2015}, volume={34}, pages={1239-1251} }