A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs.

@article{Matthews1991AHE,
  title={A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs.},
  author={G M Matthews and Alan Colman},
  journal={Nucleic acids research},
  year={1991},
  volume={19 23},
  pages={6405-12}
}
We describe the use of a Xenopus laevis egg extract for the in vitro translation and post translational modification of membrane and secretory proteins. This extract is capable of the translation and segregation into membranes of microgram per millilitre levels of protein from added mRNAs. Signal sequences of segregated proteins are efficiently cleaved and appropriate N-linked glycosylation patterns are produced. The extract also supports the quantitative assembly of murine immunoglobulin heavy… CONTINUE READING

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