A highly active synthetic mammalian retrotransposon

@article{Han2004AHA,
  title={A highly active synthetic mammalian retrotransposon},
  author={Jeffrey Han and Jef D. Boeke},
  journal={Nature},
  year={2004},
  volume={429},
  pages={314-318}
}
LINE-1 (L1) elements are retrotransposons that comprise large fractions of mammalian genomes. Transcription through L1 open reading frames is inefficient owing to an elongation defect, inhibiting the robust expression of L1 RNA and proteins, the substrate and enzyme(s) for retrotransposition. This elongation defect probably controls L1 transposition frequency in mammalian cells. Here we report bypassing this transcriptional defect by synthesizing the open reading frames of L1 from synthetic… CONTINUE READING
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