A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation.

@article{Ou2011AHS,
  title={A high-throughput screening system for Arabidopsis transcription factors and its application to Med25-dependent transcriptional regulation.},
  author={Bin Ou and Kang-Quan Yin and Sai-nan Liu and Yan Yang and Tren Gu and Jennifer Man Wing Hui and Li Zhang and Jin Ya Miao and Youichi Kondou and Minami Matsui and Hong-Ya Gu and Li-Jia Qu},
  journal={Molecular plant},
  year={2011},
  volume={4 3},
  pages={546-55}
}
The activities of transcription factors (TFs) require interactions with specific DNA sequences and other regulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and yeast-two-hybrid (Y2H) methods. The efficiency of the system was validated by examining two well-characterized TF-DNA and TF-protein… CONTINUE READING

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