A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors.

@article{Yu2008AHS,
  title={A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors.},
  author={Huimin Yu and Keith E. J. Tyo and Hal S. Alper and Daniel Klein-Marcuschamer and Gregory Stephanopoulos},
  journal={Biotechnology and bioengineering},
  year={2008},
  volume={101 4},
  pages={788-96}
}
Hyaluronic acid (HA) is an important biomaterial with functional medical and cosmetic applications. As its synthesis has been recently reported in recombinant bacteria, it is of interest to develop a high throughput screening method for the rapid isolation of HA accumulating strains transformed by combinatorial libraries. Here we report a novel two-step screening strategy to select for better HA-producing recombinant Escherichia coli strains transformed by mutation libraries of rpoD and rpoS… CONTINUE READING

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