A high-throughput pipeline for the production of synthetic antibodies for analysis of ribonucleoprotein complexes.

@article{Na2016AHP,
  title={A high-throughput pipeline for the production of synthetic antibodies for analysis of ribonucleoprotein complexes.},
  author={Hong Na and John Laver and Jouhyun Jeon and Fateh Singh and Kristin Ancevicius and Yujie Fan and Wen Xi Cao and Kun Nie and Zhenglin Yang and Hua Luo and Miranda Wang and Olivia S. Rissland and J. Timothy Westwood and Philip M. Kim and Craig A. Smibert and Howard D. Lipshitz and Sachdev S. Sidhu},
  journal={RNA},
  year={2016},
  volume={22 4},
  pages={
          636-55
        }
}
Post-transcriptional regulation of mRNAs plays an essential role in the control of gene expression. mRNAs are regulated in ribonucleoprotein (RNP) complexes by RNA-binding proteins (RBPs) along with associated protein and noncoding RNA (ncRNA) cofactors. A global understanding of post-transcriptional control in any cell type requires identification of the components of all of its RNP complexes. We have previously shown that these complexes can be purified by immunoprecipitation using anti-RBP… 
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