A high-throughput cloning system for reverse genetics in Trypanosoma cruzi

  title={A high-throughput cloning system for reverse genetics in Trypanosoma cruzi},
  author={Michel Batista and Fabricio Kerrynton Marchini and Paola AF Celedon and Stenio Perdig{\~a}o Fragoso and Christian Macagnan Probst and Henrique Preti and Luiz Shozo Ozaki and Gregory A. Buck and Samuel Goldenberg and Marco A. Krieger},
  booktitle={BMC Microbiology},
The three trypanosomatids pathogenic to men, Trypanosoma cruzi, Trypanosoma brucei and Leishmania major, are etiological agents of Chagas disease, African sleeping sickness and cutaneous leishmaniasis, respectively. The complete sequencing of these trypanosomatid genomes represented a breakthrough in the understanding of these organisms. Genome sequencing is a step towards solving the parasite biology puzzle, as there are a high percentage of genes encoding proteins without functional… CONTINUE READING


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