A high-throughput chromatin immunoprecipitation approach reveals principles of dynamic gene regulation in mammals.

@article{Garber2012AHC,
  title={A high-throughput chromatin immunoprecipitation approach reveals principles of dynamic gene regulation in mammals.},
  author={M. Garber and Nir Yosef and Alon Goren and Raktima Raychowdhury and Anne Thielke and Mitchell Guttman and James Robinson and Brian Minie and Nicolas Chevrier and Zohar Itzhaki and Ronnie Blecher-Gonen and Chamutal Bornstein and Daniela B Amann-Zalcenstein and Assaf Weiner and Dennis Friedrich and James C. Meldrim and Oren Ram and Christine Cheng and Andreas Gnirke and Sheila E Fisher and Nir Friedman and Bang Wong and Bradley E. Bernstein and Chad Nusbaum and Nir Hacohen and Aviv Regev and Ido Amit},
  journal={Molecular cell},
  year={2012},
  volume={47 5},
  pages={810-22}
}
Understanding the principles governing mammalian gene regulation has been hampered by the difficulty in measuring in vivo binding dynamics of large numbers of transcription factors (TF) to DNA. Here, we develop a high-throughput Chromatin ImmunoPrecipitation (HT-ChIP) method to systematically map protein-DNA interactions. HT-ChIP was applied to define the dynamics of DNA binding by 25 TFs and 4 chromatin marks at 4 time-points following pathogen stimulus of dendritic cells. Analyzing over 180… CONTINUE READING
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