A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae.

  title={A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae.},
  author={Wei Chen and Chengwu Zhang and Lirong Song and Milton R. Sommerfeld and Qiang Hu},
  journal={Journal of microbiological methods},
  volume={77 1},
Isolation of high neutral lipid-containing microalgae is key to the commercial success of microalgae-based biofuel production. The Nile red fluorescence method has been successfully applied to the determination of lipids in certain microalgae, but has been unsuccessful in many others, particularly those with thick, rigid cell walls that prevent the penetration of the fluorescence dye. The conventional "one sample at a time" method was also time-consuming. In this study, the solvent dimethyl… CONTINUE READING
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