A high temperature transfer procedure for detection of G protein gamma subunits by immunoblotting.

@article{Robishaw1993AHT,
  title={A high temperature transfer procedure for detection of G protein gamma subunits by immunoblotting.},
  author={Janet D. Robishaw and E A Balcueva},
  journal={Analytical biochemistry},
  year={1993},
  volume={208 2},
  pages={283-7}
}
The production of antibodies against synthetic peptides derived from amino acid sequences common or unique to a particular protein(s) is an important tool in the identification of structurally related members of an ever-increasing number of protein families. The successful use of anti-peptide antibodies requires that the protein(s) of interest be properly transferred and fully denatured prior to detection by Western-type immunoblotting. In this paper, we demonstrate that conventional transfer… CONTINUE READING