A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein

@article{Nakai2001AHS,
  title={A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein},
  author={Junichi Nakai and Masamichi Ohkura and Keiji Imoto},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={137-141}
}
Recently, several groups have developed green fluorescent protein (GFP)-based Ca2+ probes. [...] Key Result G-CaMP showed an apparent Kd for Ca2+ of 235 nM. Association kinetics of Ca2+ binding were faster at higher Ca2+ concentrations, with time constants decreasing from 230 ms at 0.2 μM Ca2+ to 2.5 ms at 1 μM Ca2+. Dissociation kinetics (τ ∼200 ms) are independent of Ca2+ concentrations.Expand
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