A high-sensitivity ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry (UHPLC-HR-TOFMS) method for screening synthetic cannabinoids and other drugs of abuse in urine

  title={A high-sensitivity ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry (UHPLC-HR-TOFMS) method for screening synthetic cannabinoids and other drugs of abuse in urine},
  author={Mira Sundstr{\"o}m and Anna Pelander and Verena Angerer and Melanie Hutter and Stefan Kneisel and Ilkka Ojanper{\"a}},
  journal={Analytical and Bioanalytical Chemistry},
AbstractThe continuing emergence of designer drugs imposes high demands on the scope and sensitivity of toxicological drug screening procedures. An ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry (UHPLC-HR-TOFMS) method was developed for screening and simultaneous confirmation of both designer drugs and other drugs of abuse in urine samples in a single run. The method covered selected synthetic cannabinoids and cathinones, amphetamines, natural… 

Comparison between drug screening by immunoassay and ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry in post-mortem urine.

The objective was to compare the performances of immunoassay and UHPLC-HR-TOF-MS in terms of scope, flexibility, sensitivity, and reliability of substance identification and to assess the effectiveness of the future option of adjusting all compound-specific reporting parameters individually.

Analysis of new designer drugs and common drugs of abuse in urine by a combined targeted and untargeted LC-HR-QTOFMS approach

The development of a liquid chromatography high-resolution mass spectrometry quadrupole-time-of-flight method for the analysis of new stimulant designer drugs and common drugs of abuse in urine is reported, providing a very useful tool for the combined targeted and untargeted analysis of drugs ofabuse in biological matrices such as urine.

Sensitive screening of synthetic cannabinoids using liquid chromatography quadrupole time-of-flight mass spectrometry after solid phase extraction.

LC-QTOF-MS was able to detect substances above trace quantities in most cases, therefore fulfilling its purpose as a sensitive general screening approach, and enables future additions for up to thousands of targets.

Broad Screening and Identification of Novel Psychoactive Substances in Plasma by High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching.

The method was proven to be suitable for the screening of additional substances and a post-run library matching was conducted for every sample with an in-house database containing over 300 NPS and known metabolites, in order to obtain a broad screening of NPS in biological matrices.

Solid-phase extraction-liquid chromatography-tandem mass spectrometry method for the qualitative analysis of 61 synthetic cannabinoid metabolites in urine.

The developed method enables the rapid and sensitive detection of synthetic cannabinoid metabolites in urine, complementing the spectrum of existing analytical tools in forensic case work.

Application of ultrasound-assisted liquid-liquid micro extraction coupled with gas chromatography-mass spectrometry for the rapid determination of synthetic cannabinoids and metabolites in biological samples.

The developed assay is highly applicable to laboratories with limited instrumental availability, thanks to the use of efficient and low-cost sample preparation and instrumental equipment, and may contribute to enhance the detection of NPS in clinical and forensic toxicology laboratories.



Evaluation of a direct high-capacity target screening approach for urine drug testing using liquid chromatography-time-of-flight mass spectrometry.

Validation of LC-TOF-MS screening for drugs, metabolites, and collateral compounds in forensic toxicology specimens.

This method represents a rapid, enhanced screen for blood and urine specimens in postmortem, driving under the influence, and drug facilitated sexual assault forensic toxicology casework.

Toxicological screening of urine for drugs by liquid chromatography/time-of-flight mass spectrometry with automated target library search based on elemental formulas.

A novel approach for utilizing liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) in qualitative screening analysis with the additional advantage of metabolite identification without actual reference substances is described.

Simultaneous analysis of several synthetic cannabinoids, THC, CBD and CBN, in hair by ultra-high performance liquid chromatography tandem mass spectrometry. Method validation and application to real samples.

The present method for the determination of several cannabinoids in hair proved to be simple, fast, specific and sensitive and was successfully applied to the analysis of 179 real samples collected from proven consumers of Cannabis, among which 14 were found positive to at least one synthetic cannabinoid.

Isotopic pattern and accurate mass determination in urine drug screening by liquid chromatography/time-of-flight mass spectrometry.

The results show that isotopic pattern matching by SigmaFit is a powerful means of identification in addition to accurate mass measurement.

Combined drug screening and confirmation by liquid chromatography time-of-flight mass spectrometry with reverse database search

The presented method enables simultaneous screening and confirmation whenever at least one qualifier ion is available, as applying an accurate mass precursor ion and one product ion surpasses the standard of four identification points that is required by the current EU protocol.

Development and practical application of a library of CID accurate mass spectra of more than 2,500 toxic compounds for systematic toxicological analysis by LC–QTOF-MS with data-dependent acquisition

In conclusion, LC–QTOF-MS in data-dependent acquisition mode combined with an accurate mass database and CID spectra library seemed to be one of the most efficient tools for systematic toxicological analysis.

Analysis of 30 synthetic cannabinoids in serum by liquid chromatography-electrospray ionization tandem mass spectrometry after liquid-liquid extraction.

A significant shift of the market of synthetic cannabinoids towards substances featuring a higher CB(1) binding affinity is demonstrated and clearly emphasize that the analysis of synthetic cannabinoid in serum or blood samples requires highly sensitive analytical methods covering a wide spectrum of substances.