A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)

@inproceedings{Li2017AHC,
  title={A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)},
  author={Chao Li and Turgay Unver and Baohong Zhang},
  booktitle={Scientific reports},
  year={2017}
}
The complex allotetraploid genome is one of major challenges in cotton for repressing gene expression. Developing site-specific DNA mutation is the long-term dream for cotton breeding scientists. The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is emerging as a robust biotechnology for targeted-DNA mutation. In this study, two sgRNAs, GhMYB25-like-sgRNA1 and GhMYB25-like-sgRNA2, were designed in the identical genomic regions of… CONTINUE READING
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The authors declare no competing financial interests

B. H. Zhang, R. Feng, +7 authors wrote the manuscript. All authors reviewed the manuscrip Interests
How to cite this article: Li, C. et al. A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.). Sci. Rep. 7, 43902; doi: 10.1038/srep43902 • 2017

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